Development of AFLP markers associated with zucchini yellow mosaicvirus resistance in cucumber (Cucumis sativus L.)

Authors : Hasan Özgür ŞIĞVA, Ahmet Fikret FIRAT, Gülden HAZARHUN, Ahmet İPEK

Pages : 982-987

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Zucchini yellow mosaic virus insert ignore into journalissuearticles values(ZYMV); is one of the most important pathogens that cause significant yield losses in many cucurbit crops including cucumber insert ignore into journalissuearticles values(Cucumis sativus L);. ZYMV resistance in cucumber is inherited by a single recessive gene. The purpose of this study was to identify molecular markers linked to the gene conferring ZYMV resistance in cucumber. We developed a population of 188 F2 plants derived from inbred cucumber lines. Individual F2 plants were self-pollinated to generate F3 populations. Ten randomly selected plants from each F3 population were tested for ZYMV resistance. We used a bulk segregant analysis method to identify putative molecular markers linked to ZYMV resistance. Using bulked DNA samples with parental lines and F1, a total of 170 sequence-related amplified polymorphism insert ignore into journalissuearticles values(SRAP);, 586 simple sequence repeat insert ignore into journalissuearticles values(SSR);, and 308 amplified fragment length polymorphism insert ignore into journalissuearticles values(AFLP); primer combinations were screened. Neither polymorphic SRAP nor SSR markers were linked with ZYMV resistance. Among the 308 AFLP primer combinations tested, an AFLP marker in the E-ACA/MCA primer combination showed significant association among parental lines, F1, and resistant and susceptible plants. The combination of E-ACA/M-CA was achieved on parental lines, F1, and 188 F2 individuals for confirmation of the marker segregation on the F2 population. We found that the combination of E-ACA/M-CA was linked to the zym locus with 6.91 cM.
Keywords : Cucumber, Cucumis sativus L, zucchini yellow mosaic virus, molecular markers, AFLP, SSR, SRAP