Random amplified polymorphic DNA as a method to screen metal-tolerant barley (Hordeum vulgare L.) genotypes

Authors : Süleyman CENKCİ, Nevra DOĞAN

Pages : 747-756

View : 21 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Differentially responding genotypes are comparatively used to understand mechanisms of metal tolerance in higher plants. The feasibility of the random amplified polymorphic DNA insert ignore into journalissuearticles values(RAPD); technique was evaluated to screen for metal tolerance within plants as an alternative to physiological growth parameters. Eight hydroponically grown barley insert ignore into journalissuearticles values(Hordeum vulgare L.); genotypes were exposed to different concentrations insert ignore into journalissuearticles values(75, 150, and 225 μM); of cadmium and copper for 7 days and subsequently harvested for determining metal tolerance indices by comparing dry biomass to genomic template stabilities based on their RAPD profiles. Dose- and genotype-dependent reductions in dry weights of shoot and root tissues were detected for both metal treatments. The tolerance indices showed that Aydanhanım/Bülbül-89 and Başgül/Tarm-92 were the most tolerant/sensitive barley genotypes to cadmium and copper, respectively. For untreated barley genotypes, the amplified RAPD bands were genotype-dependent and estimated genetic similarity coefficients ranged from 0.56 to 0.82. Metal exposure caused appearance and/or disappearance of RAPD bands in comparison to the control RAPD profiles. The alterations in RAPD profiles were dose- and genotype-dependent for the cadmium and copper treatment groups. Consequently, genomic template stability insert ignore into journalissuearticles values(GTS, %); of all barley genotypes was reduced with metal exposure. Based on GTS values, the most tolerant/sensitive barley genotypes to cadmium and copper were Aydanhanım/Tarm-92 and Avcı-2002/Orza-96, respectively. The growth and RAPD results clearly indicated that Aydanhanım, Avcı-2002, and Başgül were more tolerant, while Bülbül-89, Orza-96, and Tarm-92 were more sensitive barley genotypes to both metals. The RAPD technique could be used to screen metal-tolerant plant genotypes as an alternative method.
Keywords : Barley, cadmium, copper, genomic template stability, tolerance index