Relationships between some endogenous signal compounds and the antioxidantsystem in response to chilling stress in maize (Zea mays L.) seedlings

Authors : NEVZAT ESİM, ÖKKEŞ ATICI

Pages : 37-44

View : 15 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study investigated the correlation between endogenous levels of some signal compounds [nitric oxide insert ignore into journalissuearticles values(NO);, salicylic acid insert ignore into journalissuearticles values(SA);, abscisic acid insert ignore into journalissuearticles values(ABA);] and the antioxidant system in response to chilling stress in maize insert ignore into journalissuearticles values(Zea mays L.);. Seedlings grown for 12 or 19 days under normal conditions insert ignore into journalissuearticles values(24/20 °C); were exposed to chilling stress insert ignore into journalissuearticles values(10/7 °C); for 2 days. The levels of NO, SA, ABA, hydrogen peroxide insert ignore into journalissuearticles values(H2O2);, superoxide anion insert ignore into journalissuearticles values(O2.-);, and malondialdehyde insert ignore into journalissuearticles values(MDA); were determined in leaves of seedlings harvested on day 14 and day 21. In the same seedlings, the activities of superoxide dismutase insert ignore into journalissuearticles values(SOD);, peroxidase insert ignore into journalissuearticles values(POX);, catalase insert ignore into journalissuearticles values(CAT);, and nitrate reductase insert ignore into journalissuearticles values(NR); were analyzed. Chilling treatment enhanced the levels of NO and ABA in the seedlings harvested on both day 14 and day 21 as compared with the control group. However, SA content was elevated by chilling treatment in 14-day-old seedlings, while it did not change significantly in 21-day-old seedlings. As compared with control plants, chilling treatment increased not only antioxidant enzymes activities but also contents of H2O2, O2.-, and MDA. NR regarding ABA and NO biosynthesis was increased by chilling in maize. The results showed that chilling stress caused oxidative damage, and changes in signal molecules insert ignore into journalissuearticles values(NO, SA, and ABA); and NR activity in maize.
Keywords : Chilling stress, nitric oxide, salicylic acid, abscisic acid, oxidative damage, antioxidant enzyme, HPLC