Genetic structure and diversity of Adonis L. (Ranunculaceae) populations collected from Turkey by inter-primer binding site (iPBS) retrotransposon markers

Authors : Arash Hossein POUR, Faruk KARAHAN, Emre İLHAN, Ahmet İLÇİM, Kamil HALİLOĞLU

Pages : 585-596

View : 20 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The genus Adonis L. is a member of Ranunculaceae and consists of perennial and annual herbaceous plants included in the tribe Adonideae under the subfamily Ranunculoideae. Botanically, Ranunculaceae comprises vital medicinal plants. Molecular markers are one of the most effective tools for exploring genetic variation that can enhance breeding efficiency. To identify the genetic diversity of 62 Adonis ecotypes collected from different regions in Turkey, the interprimer binding site insert ignore into journalissuearticles values(iPBS); retrotransposon system was used. Of the 83 iPBS primers used, 10 provided sufficient polymorphic data, generating a total of 204 alleles. The number of iPBS bands per individual was 3.29, and the number of alleles per polymorphic locus ranged from 8 to 35, with an average of 20.30. The average polymorphism percentage was 99.50%, and polymorphic information content ranged from 0.16 to 0.39. The highest average number of alleles, Nei`s genetic diversity insert ignore into journalissuearticles values(h);, and Shannon`s information index insert ignore into journalissuearticles values(I); were obtained from A. volgensis species insert ignore into journalissuearticles values(1.64, 0.39, and 0.58, respectively);, whereas the lowest values insert ignore into journalissuearticles values(1.41, 0.29, and 0.46, respectively); were found in A. flammea species. The analysis of molecular variance revealed significant variance within the population insert ignore into journalissuearticles values(71%);, whereas no significant genetic variation was observed among the different species insert ignore into journalissuearticles values(29%);. Cluster analysis according to unweighted pair-group mean average insert ignore into journalissuearticles values(UPGMA); divided 62 Adonis ecotypes into four major clusters. According to the principal coordinate analysis, the first three principal coordinates accounted for 81.51% of total variation. Genetic structure analysis of the studied germplasm using the Bayesian method revealed four subpopulations with an average of 0.2634 for expected heterozygosity and 0.2154 for population differentiation measurements. The results of this study suggested that iPBS markers could be used in the identification of genetic diversity among the Adonis species.
Keywords : Adonis L, iPBS markers, genetic diversity, population structure, AMOVA