Acquisition of boron tolerance by salt pretreatment in two sunflower cultivars

Authors : Yasemin EKMEKÇİ, Şeküre ÇULHA ERDAL, Ayşe Suna BALKAN NALÇAİYİ, Nuran ÇİÇEK

Pages : 153-166

View : 23 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study was carried out to investigate the ameliorative effects of salt pretreatment against B toxicity in two sunflower cultivars insert ignore into journalissuearticles values(Helianthus annuus L. `Sanbro? and `Tarsan-1018?); differing in salt tolerance. Seedlings were grown in perlite with modified half-strength Hoagland?s solution for 15 days and then they were divided into two groups: salt-pretreated insert ignore into journalissuearticles values(75 mM NaCl for 5 days); and B-treated insert ignore into journalissuearticles values(control, 2, 4, and 8 mM B for 10 days);. In both cultivars, the biomass of root and shoot decreased depending on B accumulation, especially at 8 mM. The translocation factor values indicated that B uptaken by roots of the genotypes were translocated to the leaves. High B accumulation adversely affected the water balance and membrane integrity of the leaves. Additionally, toxic B levels caused changes in the some JIP tests and slow fluorescence parameters insert ignore into journalissuearticles values(ABS/RC, TR0/RC, ET0/RC, RE0/RC, DI0/RC, Area, ?E0, ?R0 and jD0, ?PSII, ETR); of both cultivars and these changes led to a significant decrease in photosynthetic performance insert ignore into journalissuearticles values(PIABS and PITOTAL);. Salt pretreatment ameliorated the damaging effects of toxic B on membrane integrity, water content, and the photosynthetic process; decreased B accumulation; and improved the membrane stability. Both cultivars acquired tolerance against B toxicity with salt pretreatment and survived in increasing boron toxicity. We conclude that sunflower can be used for phytoremediation purposes for boron-contaminated soils. Additionally, this study is the first report to reveal that moderate salt stress pretreatment alleviates B toxicity and provides tolerance to B. This alleviation might be achieved by NaCl to decrease the boron uptake from the roots.
Keywords : Boron toxicity, JIP test, photochemical activity, salt pretreatment, slow and fast chlorophyll a fluorescence, sunflower Helianthus annuus L,