In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes

Authors : Büşra Koncuk CEBECİ, Zuhal ALIM, Şükrü BEYDEMİR

Pages : 512-520

Doi:10.3906/kim-1308-20

View : 22 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The organophosphate target enzyme paraoxonase-1 insert ignore into journalissuearticles values(PON1); has been extensively studied in toxicology. Pesticides are organophosphate compounds that are commonly used in agriculture. They are also used as nerve gases, such as sarin and soman. Therefore, the effects of these compounds on enzymatic activity are well known. In the present study, we investigated the in vitro effects of fenoxaprop-p-ethyl, lambda cyhalothrin, imidacloprid, and dichlorvos pesticides on sheep liver microsomal PON1. The enzyme was purified approximately 141-fold with a specific activity of 1822.22 EU/mg proteins. The pesticides inhibited sheep liver PON1 in vitro, and the IC50 values for these compounds were 0.0103, 0.069, 0.157, and 0.2 m M, respectively.
Keywords : Paraoxonase, fenoxaprop p ethyl, lambda cyhalothrin, imidacloprid, dichlorvos, inhibition