Sesquiterpene hydrocarbons of the essential oil of Actinolema macrolema Boiss

Authors : Betül DEMİRCİ, Gülden Koltuksuz YASDIKCIOĞLU, Kemal Hüsnü Can BAŞER

Pages : 917-926

Doi:10.3906/kim-1103-24

View : 22 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The essential oils of Actinolema macrolema Boiss. insert ignore into journalissuearticles values(Apiaceae); were obtained by hydrodistillation in the first 3 insert ignore into journalissuearticles values(sample A); and the following 3 h insert ignore into journalissuearticles values(sample B); from crushed fruits and dried leaves insert ignore into journalissuearticles values(sample C);, which were subsequently analyzed by GC and GC-MS. Overall, 64 components were characterized, representing 93% of the leaf oil. Thirty components were characterized from the fruit oil representing 95% of the first fraction and 90% of the second fraction consecutively. Guaia-5,7insert ignore into journalissuearticles values(11);-diene, selina-3,7insert ignore into journalissuearticles values(11);-diene, and juniper camphor were isolated from the oils by column chromatography and their structures were elucidated by GC-MS, 1H NMR, and 13C NMR. The occurrence of guaia-5,7insert ignore into journalissuearticles values(11);-diene in nature is reported for the first time. Guaia-5,7insert ignore into journalissuearticles values(11);-diene insert ignore into journalissuearticles values(37% and 30%);, germacrene-B insert ignore into journalissuearticles values(25% and 21%);, and selina-3,7insert ignore into journalissuearticles values(11);-diene insert ignore into journalissuearticles values(both 12%); were found as major components in the oil of sample A and the following sample B, respectively. In sample C, 1-octadecanol insert ignore into journalissuearticles values(24%); and hexadecanoic acid insert ignore into journalissuearticles values(19%); were identified as the major components. Additionally, antimicrobial activities of the fruit oils were determined using broth microdilution. Sample A exhibited relatively good inhibition of Staphylococcus epidermidis insert ignore into journalissuearticles values(MIC 62.5 m g/mL);. The 2 fruit essential oils showed inhibitory insert ignore into journalissuearticles values(MIC 125 m g/mL); effects equal to those of the standard antifungal agent used against C. albicans.
Keywords : Actinolema macrolema, Apiaceae, essential oil, isolation, guaia 5, 7 11, diene, GC MS, biological activity