Optimization of a gas chromatography--mass spectrometry method using chemometric techniques for the determination of ezetimibe in human plasma

Authors : Ebru UÇAKTÜRK, Nuran ÖZALTIN

Pages : 734-745

Doi:10.3906/kim-1210-18

View : 14 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A new, rapid, and sensitive gas chromatography--mass spectrometry insert ignore into journalissuearticles values(GC-MS); method was developed for the determination of ezetimibe insert ignore into journalissuearticles values(EZE); in human plasma. EZE was derivatized prior to GC-MS analysis. Various derivatization techniques such as acetylation, methylation, and silylation were tried. EZE was extracted from plasma with high recovery insert ignore into journalissuearticles values(94.39%--97.57%); using methyl tertbutyl ether and carbonate buffer insert ignore into journalissuearticles values(pH 9);. Chromatographic conditions were optimized using chemometric methods. In the first step, optimization with factorial design, chromatographic variables insert ignore into journalissuearticles values(initial and final column temperature, oven ramp rate, and flow rate of gas); were screened to select important variables for the retention of EZE. In the second step, central composite design was applied to decide on the retention time of EZE. The analysis was achieved in a short period of time insert ignore into journalissuearticles values(
Keywords : Ezetimibe, gas chromatography mass spectrometry, human plasma, experimental design, validation