99mTc labeled levofloxacin as an infection imaging agent: a novel method for labeling levofloxacin using cysteine\cdot HCl as co-ligand and in vivo study

Authors : Syed Ali Raza NAQVI, Malik Muhammad ISHFAQ, Zulfiqar Ali KHAN

Pages : 267-277

Doi:10.3906/tar-1203-71

View : 14 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Levofloxacin was labeled with 99mTc using cysteine\cdot HCl as co-ligand and SnCl2.2H2O as reducing agent. The influence of various parameters such as amount of cysteine\cdot HCl, reducing agent, pH value, and reaction time on labeling process was studied. After optimizing the conditions the labeling was performed at pH 5 using 1 mg of levofloxacin, 500 m g of cysteine\cdot HCl, 50 m g of SnCl2.2H2O, and 15 min reaction time. The radiochemical purity was determined with the help of instant thin layer chromatography insert ignore into journalissuearticles values(ITLC); and reverse phase high performance liquid chromatography insert ignore into journalissuearticles values(RP-HPLC); which was more than 95% and was stable for up to 6 h. Biodistribution of 99mTc-levofloxacin insert ignore into journalissuearticles values(99mTc-lefx); was studied in infection induced rat models using live Staphylococcus aureus and heat killed S. aureus insert ignore into journalissuearticles values(sterile inflammation model);. In the case of the live S. aureus induced abscess model, the accumulation of 99mTc-lefx at target was 3.96, which was higher than that of 99mTc-ciprofloxacin insert ignore into journalissuearticles values(99mTc-cifx);, taken as the control.
Keywords : Infection, 99mTc levofloxacin, co ligand, scintigraphy, biodistribution, infection model