Constituents of Plantago major subsp. intermedia with antioxidant and anticholinesterase capacities

Authors : Ufuk KOLAK, Mehmet BOĞA, Emine Akalin URUŞAK, Ayhan ULUBELEN

Pages : 637-645

Doi:10.3906/sag-1211-14

View : 20 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The methanol extract of Plantago major subsp. intermedia insert ignore into journalissuearticles values(Gilib.); Lange afforded 4 known compounds, namely isomartynoside insert ignore into journalissuearticles values(1);, 10-hydroxymajoroside insert ignore into journalissuearticles values(2);, b -sitosterol insert ignore into journalissuearticles values(3);, and ursolic acid insert ignore into journalissuearticles values(4);. Their structures were established by spectroscopic methods. After determination of the total phenolic and flavonoid contents of the methanol extract, the antioxidant potentials of the crude extract and isolated compounds 1-4 were determined by b -carotene bleaching, DPPH free radical and ABTS cation radical scavenging, and cupric reducing antioxidant capacity methods. The methanol extract, rich in phenolic contents, indicated the same DPPH free radical scavenging activity insert ignore into journalissuearticles values(72% inhibition); as a standard compound, butylated hydroxytoluene, at 100 m g/mL. Isomartynoside insert ignore into journalissuearticles values(1);, a phenylpropanoid glycoside, showed the best inhibition of lipid peroxidation, ABTS cation radical scavenging activity, and cupric reducing antioxidant capacity among the tested samples. The anticholinesterase effects of the methanol extract and isolated compounds 1-4 were established using the Ellman method. A triterpenic acid, ursolic acid insert ignore into journalissuearticles values(4);, exhibited moderate acetyl- insert ignore into journalissuearticles values(54.01 \pm 0.82%); and butyryl-cholinesterase insert ignore into journalissuearticles values(68.74 \pm 0.36%); inhibitory activity at 200 m g/mL. Isomartynoside insert ignore into journalissuearticles values(1); was isolated here for the first time from a Plantago species; the antioxidant and anticholinesterase activities of P. major subsp. intermedia and compounds 1-2 were not previously determined.
Keywords : Plantago major subsp intermedia, Plantaginaceae, isomartynoside, phenylpropanoid glycoside, antioxidant, anticholinesterase