A calorimetric study of the interaction of silver ions with jack bean urease

Authors : Ali Akbar SABOURY, Elahe POORAKBAR, Gholamreza Rezaei BEHBEHANI

Pages : 631-638

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A thermodynamic study of silver ions by jack bean urease insert ignore into journalissuearticles values(JBU); was carried out at 2 temperatures, 27 and 37 °C, in Tris buffer insert ignore into journalissuearticles values(30 mM; pH 7.0); using isothermal titration calorimetry insert ignore into journalissuearticles values(ITC);. There was a set of 12 identical and noninteracting binding sites for the silver ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding were 185 m M and -16.7 kJ mol-1 at 27 °C, and 229 m M and -16.3 kJ mol-1 at 37 °C, respectively. The molar entropy of binding was +15.7 J K-1 mol-1 at 27 °C and +17.1 J K-1 mol-1 at 37 °C. Hence, the binding process of silver ions to JBU is not only enthalpy driven but is also entropy driven, and the role of entropy should be made more effective by increasing the temperature.
Keywords : Urease, silver ion, isothermal titration calorimetry, binding constant, enthalpy of binding, entropy of binding