Synthesis and Cloning of a Small Bacillus Pheromone Gene (ComXRO-B-2) by Primer-Dimer Formation with PCR

Authors : Devrim DEMİR DORA, Tanıl KOCAGÖZ, Filiz ÖNER

Pages : 765-771

View : 14 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :ComX pheromone is the major extracellular signaling peptide stimulating transformation in response to high cell density in Bacillus species. In this study, Bacillus mojavensis ComXinsert ignore into journalissuearticles values(RO-B-2); pheromone gene was cloned to pGEMT-Easy vector based on TA cloning of PCR products. The gene encoding 11 amino acid peptides of Bacillus mojavensis RO-B-2 strain ComX pheromone insert ignore into journalissuearticles values(GLQIYTNWVPS); was obtained by PCR amplification of 2 primers complementary to each other at their 3` end. This eliminated the need for the original bacterial gene as DNA template for PCR. The amplified PCR products were ligated directly without any modification by T4 DNA ligase into pGEMT-Easy vector, which has a single overhanging T residue at the 3` ends of the cloning site.
Keywords : Bacillus mojavensis RO B 2, ComX pheromone gene, TA cloning, primer dimer, PCR