Performance Comparison between Monolithic C18 and Conventional C18 Particle-Packed Columns in the Liquid Chromatographic Determination of Propranolol HCl

Authors : Sami El DEEB, Hermann WÄTZIG

Pages : 543-552

View : 19 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Monolithic and conventional particle-packed columns were applied for the determination of propranolol hydrochloride in the presence of its 2 main degradation products, 3-insert ignore into journalissuearticles values(1-naphthyloxy);-propane-1,2- diol and 4-isopropyl-1,7-bis-insert ignore into journalissuearticles values(1-naphthyloxy);-4-azaheptane-2,6-diol. The separations were investigated on monolithic columns at flow rates from 1 to 9 mL/min. Fast and efficient separation was obtained by monolithic columns. The analysis time was decreased by about 5-fold on monolithic columns at a flow rate of 4 mL/min, while maintaining sufficient resolution between propranolol and its degradation products. The method was validated using a set of 3 monolithic columns and compared to a conventional insert ignore into journalissuearticles values(Superspher); C18 column. The precision for both retention time and peak area was investigated over a wide concentration range insert ignore into journalissuearticles values(0.002- 1 mg/mL); and found to be equal or slightly better on Chromolith Performance compared to the conventional column. Batch to batch reproducibility of the Chromolith Performance columns insert ignore into journalissuearticles values(n = 3); was also calculated. The RSDs % equal 0.66% for retention time and ranged from 0.45% to 1.12% for peak areas. Practical parameters including the pressure drop, plate height, retention time and resolution of monolithic columns were compared to those of a conventional insert ignore into journalissuearticles values(Superspher); C18 column. The detection and quantitation limits on monolithic columns at both flow rates insert ignore into journalissuearticles values(1 and 4 mL/min); were 0.012 and 0.04 m g/mL, compared with 0.061 and 0.2 m g/mL on the conventional column. The method showed good linearity and recovery and was found to be suitable for the analysis of propranolol hydrochloride formulations.
Keywords : Monolithic columns, Chromolith Performance, propranolol hydrochloride, performance, validation, degradation products, method transfer, batch reproducibility