The Simultaneous Determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in Syrups and Tablets by Second Derivative Spectrophotometry

Authors : Mahmure ÜSTÜN ÖZGÜR

Pages : 501-508

View : 13 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A very simple spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of the food dyes, Sunset Yellow insert ignore into journalissuearticles values(E-110); and Quinoline Yellow insert ignore into journalissuearticles values(E-104);. Calibration graphs are linear up to 15.0 m gml-1 of Sunset Yellow insert ignore into journalissuearticles values(r = 0.9998);, and Quinoline Yellow insert ignore into journalissuearticles values(r = 0.9999);. The assay procedure for E-110 and E-104 in pharmaceutical products involves the extraction of the dyes with AcOH/NaOAc buffer solution insert ignore into journalissuearticles values(pH = 4.5);, filtration, and measurement of the second derivative absorbance values at 410.0 nm for E-104 and 533.1 nm for E-110. This method was used for determining synthetic mixtures of these dyes in different ratios and it was successfully applied to two commercial products without a previous separation step.
Keywords : Dyes, derivative spectrophotometry, pharmaceutical products, Sunset Yellow, Quinoline Yellow