Incorporation of EDTA for the Elimination of Metal Inhibitory Effects in an Amperometric Biosensor Based on Mushroom Tissue Polyphenol Oxidase

Authors : Arzum ERDEM, Dilşat ÖZKAN, Burcu MERİÇ, Kağan KERMAN

Pages : 231-239

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Enzyme based biosensors are highly selective devices which rely on the specific binding of the target analyte insert ignore into journalissuearticles values(the substrate); to the active-site regions of the enzyme. The enzymatic reaction between mushroom tissue polyphenol oxidase and its substrate phenol is coupled with the use of potassium ferrocyanide insert ignore into journalissuearticles values(K4Feinsert ignore into journalissuearticles values(CN);6); as the mediator in ammonia buffer solution insert ignore into journalissuearticles values(pH 8.80);. The response of devices is often affected by the presence of inhibitors, which combine with the free enzyme in a manner that prevents substrate binding. The objective of this study is to describe an efficient and yet simple strategy to eliminate the interference of metals in amperometric biosensing. The methods relies on in situ removal of the interfering metal by a surface-bound complexing agent, disodium ethylenediaminetetraacetate insert ignore into journalissuearticles values(EDTA);. EDTA is incorporated into the electrode by mixing it within the enzyme-containing carbon paste matrix. Mixed enzyme/carbon paste electrodes are receiving considerable attention for the preparation of fast responding biosensors.
Keywords : Turk J Chem, 25, 2001, 231 239 Turk J Chem, vol 25, iss 2