Some Properties of Cu, Zn-Superoxide Dismutase from Sheep Erythrocyte

Authors : Leman TARHAN

Pages : 109-116

View : 16 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Superoxide dismutase insert ignore into journalissuearticles values(SOD); was isolated from sheep erythrocyte. SOD activity was measured under the optimized assay conditions by observing the variations of autoxidation rate of 6-hydroxydopamine insert ignore into journalissuearticles values(6-OHDA);. The enzyme was characterized as containing copper and zinc and was insensitive to chloroform-ethanol mixture but inhibited by cyanide and hydrogen peroxide. The activity variations and stability properties of sheep erythrocyte Cu, Zn-SOD were investigated under the optimized activity assay conditions by observing inhibition change at the autoxidation rate of 6-OHDA. The optimum pH and temperature of sheep erythrocyte Cu, Zn-SOD were found to be 9.4 and 30\circC respectively. The enzyme showed high pH- and thermal-stability properties around neutral pH and up to 37\circC after 2.5 h incubation. Variations in the inhibition percentage of autoxidation were investigated in 0.2-0.9 mM range of 6-OHDA. The same procedures were repeated by adding catalase also.
Keywords : Superoxide radical, Cu, Zn superoxide dismutase, 6 hydroxydopamine, dismutation