Thin Layer Chromatographic Separation and Quantitation of L-Dopa and L-Tyrosine in Mixtures

Authors : Gülendem GÜNENDİ, Fahrünnisa PAMUK

Pages : 269-274

View : 17 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A new TLC separation and quantitation method for L-tyrosine and L-Dopa mixtures was developed. The minimum tyrosine and Dopa quantities which can be measured by this technique are 0.7 and 1.5 m g respectively. The method may be used to measure the kinetic parameters of polyphenoloxidase as well as to trace the enzyme catalyzed conversion of L-tyrosine into L-Dopa. For a set of 20 measurements the maximum difference between any two measurements of spot areas was found to be 3 mm2 insert ignore into journalissuearticles values(4.5%); for L-Dopa insert ignore into journalissuearticles values(4 m g); and 2mm2 insert ignore into journalissuearticles values(5.6%); for L-tyrosine insert ignore into journalissuearticles values(1m g);.
Keywords : TLC Separation, L Dopa L Tyrosine mixtures