Electrochemical Preparation and Sensor Properties of Conducting Polyaniline Films

Authors : M ÖZDEN, ERGÜN EKİNCİ and A ERSİN KARAGÖZLER

Pages : 89-98

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A polyaniline-glucose oxidase electrode was prepared by the electrochemical polymerization of aniline on a Pt electrode that was already pre-adsorbed by the enzyme insert ignore into journalissuearticles values(glucose oxidase); at a potential of 1 V vs. Ag/AgCl. Then, the amperometric responses of the enzyme electrode to the electroactive hydrogen peroxide formed by the enzyme-catalyzed reaction of the substrate insert ignore into journalissuearticles values(glucose); with oxygen were measured at a potential of 0.7 V in PBS solution. The effects of electrochemical polymerization insert ignore into journalissuearticles values(i.e., concentrations of monomer and electrolyte, film thickness); and amperometric measurement parameters insert ignore into journalissuearticles values(i.e. pH, temperature); on the amperometric response characteristics to glucose of the resultant enzyme electrode were systematically investigated and all these parameters were optimized. The prepared polymeric sensor exhibited a fast steady-state amperometric response time insert ignore into journalissuearticles values(4-5 s);, a linear amperometric response up to 6 mM glucose through with poor stability. Also, it was found that the sensor responded successfully to glucose injections in the presence of some interfering substances such as ascorbic acid, oxalic acid, lactose, sucrose and urea.
Keywords : Polyaniline, enzyme electrode, amperometric biosensor