Electrochemical monitoring of the interaction between mitomycin C and DNA at chitosan--carbon nanotube composite modified electrodes

Authors : Pembe Ece CANAVAR, Ece EKŞİN, Arzum ERDEM

Pages : 1-12

Doi:10.3906/kim-1402-11

View : 22 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Single-walled carbon nanotube insert ignore into journalissuearticles values(CNT); and chitosan composite insert ignore into journalissuearticles values(chitosan*CNT); based sensors were developed as DNA biosensors, and then they were applied for electrochemical investigation of the interaction between the anticancer drug mitomycin C insert ignore into journalissuearticles values(MC); and DNA. The oxidation signals of MC and guanine were monitored before and after the interaction process by differential pulse voltammetry insert ignore into journalissuearticles values(DPV);. The DPV results were in good agreement with those of electrochemical impedance spectroscopy insert ignore into journalissuearticles values(EIS);. Analytical parameters such as DNA concentration, MC concentration, and MC interaction time with DNA were optimized. The detection limits were 6.85 m g/mL for DNA and 11.01 m g/mL for MC.
Keywords : Chitosan, single walled carbon nanotubes, mitomycin C, DNA interactions, disposable graphite electrode, electrochemical impedance spectroscopy, differential pulse voltammetry