Simultaneous determination of dexpanthenol, lidocaine hydrochloride, and mepyramine maleate in combined pharmaceutical gel by capillary electrophoresis

Authors : Gülden Başmakçi AKYIL, Hayriye Eda Şatana KARA, Sezen Yarimkaya BAŞ, Nusret ERTAŞ, Nilgün GÜNDEN GÖĞER

Pages : 756-764

Doi:10.3906/kim-1309-48

View : 25 | Download : 12

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A new capillary electrophoresis method was developed for the simultaneous determination of dexpanthenol insert ignore into journalissuearticles values(DEX);, lidocaine insert ignore into journalissuearticles values(LID);, and mepyramine insert ignore into journalissuearticles values(MEP); in pharmaceutical preparations. The best results were obtained using 20 mM insert ignore into journalissuearticles values(pH 3.0); phosphate buffer as the background electrolyte. Separation was obtained using a fused-silica capillary insert ignore into journalissuearticles values(75 m m internal diameter, 50 cm total length, 41 cm effective length); and a potential of +30 kV at 20 °C. Standards and samples were injected using a pressure injection at 50 mbar for 5 s and the analytes were monitored at a detection wavelength of 200 nm. Under optimum conditions, migration times were 2.457 min for MEP, 3.520 min for LID, and 7.363 min for DEX. The method was linear over the ranges of 25--200 m g mL-1 for DEX, 7.5--60 m g mL-1 for MEP, and 7.5--60 m g mL-1 for LID. Limit of detection insert ignore into journalissuearticles values(LOD); values were 0.8 m g mL-1 for MEP, 1.8 m g mL-1 for LID, and 3.1 m g mL-1 for DEX. The developed method is accurate, precise, sensitive, selective, and repeatable.
Keywords : Capillary electrophoresis, dexpanthenol, lidocaine, mepyramine, pharmaceutical gel