Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor

Authors : Meryem TOPAL, İlhami GÜLÇİN

Pages : 894-902

Doi:10.3906/kim-1403-5

View : 21 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Rosmarinic acid is a water-soluble ester of caffeic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II insert ignore into journalissuearticles values(hCA I and II); purified from human erythrocytes by using Sepharose-4B affinity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold purification of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO2-esterase activity gave Ki values of 86.0 m M and 57.0 m M, respectively.
Keywords : Carbonic anhydrase I, carbonic anhydrase II, rosmarinic acid, enzyme purification, enzyme inhibition