Characterization of a Cu2+ -selective fluorescent probe derived from rhodamine B with 1,2,4-triazole as subunit and its application in cell imaging

Authors : Na LI, Chunwei YU, Yuxiang JI, JUN ZHANG

Pages : 660-666

Doi:10.3906/kim-1410-58

View : 14 | Download : 13

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A rhodamine B derivative containing 1,2,4-triazole as subunit was characterized as an `off-on` type Cu$^{2+}$-selective fluorescent probe. It exhibited high selectivity and sensitivity for Cu$^{2+}$ in ethanol-water solution insert ignore into journalissuearticles values(9:1, v:v, pH 7.0, 20 mM HEPES); and underwent ring opening. A prominent fluorescence enhancement at 570 nm was observed in the presence of Cu$^{2+}$ with the change in the absorption spectrum, and a 1:1 metal-ligand complex was formed. With the optimized experimental conditions, the probe exhibited a dynamic response range for Cu$^{2+}$ from 8.0 $\times $ 10$^{-7}$ to 7.5 $\times $ 10$^{-6}$ M with a detection limit of 2.3 $\times $ 10$^{-7}$ M in ethanol-water solution insert ignore into journalissuearticles values(9:1, v:v, pH 7.0, 20 mM HEPES);. Its application in Cu$^{2+}$ imaging in living cells was also studied.
Keywords : Fluorescent probe, rhodamine B, triazole, Cu ^ 2