Acid-free synthesis of S-nitrosothiols at neutral pH by shock-freezing in liquid nitrogen

Authors : DIMITRIOS TSIKAS, Dimitrios TSIKAS, ANKE BÖHMER, ARNE TRETTIN, ARSLAN ARINÇ KAYAÇELEBİ

Pages : 676-682

Doi:10.3906/kim-1501-142

View : 16 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A laboratory method for the acid-free synthesis of S-nitrosothiols insert ignore into journalissuearticles values(RSNO); from NaNO$_{2}$ and thiols in aqueous buffer of neutral pH is reported. Equimolar amounts of NaNO$_{2}$ and thiol are added to a 100-$\mu $M Na$_{4}$EDTA-containing Na$_{2}$HPO$_{4}$ buffer insert ignore into journalissuearticles values(100 mM, pH 7.4);. Samples are set into liquid nitrogen for 1 min and then thawed to room temperature or in an ice bath. Dependent upon the RSNO, repetition of the procedure may be required. This method may be of particular importance when the classical acid-based method fails or releases harmful gases such as H$_{2}$S.
Keywords : LC MS, nitric oxide, nitrite, S nitrosothiols