HPLC fluorescence determination of ochratoxin A utilizing a double internal standard and its application to poultry feed

Authors : Muzaffer TUNÇEL, Elif Mine Öncü KAYA, Ülkü Dilek UYSAL, Tufan GÜRAY

Pages : 372-0

Doi:10.3906/kim-1408-49

View : 22 | Download : 11

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A validated liquid chromatography method employing a fluorescence detector for the determination of ochratoxin A insert ignore into journalissuearticles values(OTA); was developed with double internal standard and it was applied to ten different poultry feeds. The analysis was performed in an octadecyl silane column using a solvent system [ACN:water:formic acid insert ignore into journalissuearticles values(50:50:1.25, v/v/v);] by isocratic elution. The flow rate and injection volume were 1 mL min^{-1} and 12 \mu L, respectively. Signals were detected at 278insert ignore into journalissuearticles values(\lambda_{ex});/315insert ignore into journalissuearticles values(\lambda_{em}); and 330insert ignore into journalissuearticles values(\lambda_{ex});/450insert ignore into journalissuearticles values(\lambda_{em}); nm between 0 and 8, and 8.01 and 20.0 min, respectively. The method was validated with precision, linearity, accuracy, limit of detection, limit of quantification, robustness, and stability. Good linearity insert ignore into journalissuearticles values(r^2 = 0.9998--0.9999); was achieved over a concentration range of 1.60 \times 10^{-8} M to 6.40 \times 10^{-6} M for OTA. LOD and LOQ values were 7.83 \times 10^{-10} M and 2.37 \times 10^{-9} M, and 2.01 \times 10^{-9} M and 6.10 \times 10^{-9} M for internal standard 1 insert ignore into journalissuearticles values(IS1); and internal standard 2 insert ignore into journalissuearticles values(IS2);, respectively, on an interday basis. The method was applied to poultry feed samples. Good recovery data ranged between 79.10% and 85.57%, and 71.98% and 76.66%, and the RSD% values were in the range of 1.36--11.70 and 2.07--2.34 for IS1 and IS2, respectively.
Keywords : Animal feed and poultry feed, fluorescence detector, HPLC, mycotoxin, ochratoxin A