Immobilization of laccase onto a porous nanocomposite: application for textile dye degradation

Authors : Sedef İLK, Deniz DEMİRCAN, Semran SAĞLAM, Necdet SAĞLAM, Zakir M O RZAYEV

Pages : 262-276

View : 19 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Polyinsert ignore into journalissuearticles values(MA-\textit{alt}-MVE);-$g$-PLA/ODA-MMT nanocomposite was prepared by self-catalytic interlamellar graft copolymerization of L-lactic acid insert ignore into journalissuearticles values(LA); onto polyinsert ignore into journalissuearticles values(maleic anhydride-\textit{alt}-methyl vinyl ether); copolymer in the presence of octadecyl amine-montmorillonite insert ignore into journalissuearticles values(ODA-MMT); organoclay. FTIR, $^{1}$H insert ignore into journalissuearticles values($^{13}$C); NMR, XRD, and SEM-TEM were utilized for characterizing the resultant nanocomposite. Laccase from \textit{Trametes versicolor} was immobilized onto the prepared nanocomposite by adsorption or covalent coupling. Decolorization of Reactive Red 3 from aqueous solution by laccase immobilized on the nanocomposite was studied in different conditions insert ignore into journalissuearticles values(pH, temperature, dye concentration, and reaction time); to investigate the decolorization activity with repeated use and storage. The results indicated that more than 77{\%} of the activity of laccase immobilized systems was retained at the end of 10 cycles. The final decolorization capacity of the immobilized laccase was significantly higher insert ignore into journalissuearticles values(65{\%}); than that of free laccase insert ignore into journalissuearticles values(33{\%}); in the chosen optimized conditions insert ignore into journalissuearticles values(pH 5, 20 $^{\circ}$C, 0.05 mg/mL laccase concentration, and 90 min);.
Keywords : Nanocomposite, functional copolymer, interlamellar graft copolymerization, enzyme laccase, immobilization, reactive dyes, decolorization