Voltammetric analysis of disulfiram in pharmaceuticals with a cyclic renewable silver amalgam film electrode

Authors : SYLWIA SMARZEWSKA, NATALIA FESTINGER, MONIKA SKOWRON, DARIUSZ GUZIEJEWSKI, RADOVAN METELKA, MARIOLA BRYCHT, WITOLD CIESIELSKI

Pages : 116-124

View : 17 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Electrochemical properties of disulfiram, representative of highly significant bioactive compounds, were studied with a cyclic renewable silver amalgam film electrode insert ignore into journalissuearticles values(Hginsert ignore into journalissuearticles values(Ag);FE); using square wave cathodic stripping voltammetry insert ignore into journalissuearticles values(SWCSV);. The influence of various factors such as pH, buffer concentration, buffer composition, and SWCSV parameters on current response was investigated. The optimum results in terms of signal shape and intensity were recorded in Britton--Robinson buffer insert ignore into journalissuearticles values(pH 7.5); at -0.5 V versus Ag/AgCl/3 mol L$^{-1}$ KCl. An elaborated electroanalytical procedure was used to determine disulfiram at the Hginsert ignore into journalissuearticles values(Ag);FE in the concentration range from 0.05 to 5.00 $\mu $M. Precision, repeatability, and accuracy of the method as well as the influence of possible interferences were ascertained. The detection and quantification limits were 11 nM and 37 nM, respectively. The applicability of the developed method was tested in the determination of disulfiram in the commercial formulation Anticol. Thin-layer chromatography with image processing software was used to validate the accuracy of the method.
Keywords : Disulfiram, silver amalgam electrode, square wave voltammetry, drug analysis, Anticol