A sample stacking-capillary electrophoresis method for simultaneous determination of nitrate and thiocyanate ions of ultra-heat-treated milk samples

Authors : Filiz TEZCAN

Pages : 1184-1190

View : 15 | Download : 11

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :  Ultra-heat-treated insert ignore into journalissuearticles values(UHT); cow milk is mostly consumed in Turkey. Heating milk can cause some chemical and biochemical changes that affect different components of milk. However, studies on the constituents of UHT milk are scare. In the present study, nitrate and thiocyanate contents of commercial UHT~whole cow milk were determined simultaneously by sample stacking-capillary electrophoresis. The best separation of nitrate and thiocyanate ions was performed by reducing the electroosmotic flow at pH 4.0 using 30 mM formic acid and 30 mM sodium sulfate containing separation electrolyte. Analysis was completed in less than 3 min. The limits of detection obtained at a signal-to-noise ratio of 3 for nitrate and thiocyanate were 0.03 mg/L insert ignore into journalissuearticles values(0.48 μ ` role=`presentation`> μ μ \mu mol/L); and 0.06 mg/L insert ignore into journalissuearticles values(1.03 μ ` role=`presentation`> μ μ \mu mol/L);, respectively. The nitrate concentration in commercial UHT milk samples ranged between 0.20 and 1.50 mg/L insert ignore into journalissuearticles values(3.23-24.20 μ ` role=`presentation`> μ μ \mu mol/L); and the thiocyanate ion concentration ranged between 0.41 and 0.67 mg/L insert ignore into journalissuearticles values(7.06-11.55 μ ` role=`presentation`> μ μ \mu mol/L);.
Keywords : Capillary electrophoresis, milk, nitrate, sample stacking, thiocyanate