Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase

Authors : Aysu YARMAN

Pages : 346-354

View : 14 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :For the first time a molecularly imprinted polymer insert ignore into journalissuearticles values(MIP);-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase K or by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM insert ignore into journalissuearticles values(R$^{2}$ = 0.994); and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.
Keywords : Molecularly imprinted polymers, biomimetic sensors, tyrosinase, electropolymerization, scopoletin, o phenylenediamine