A novel hypobromous acid scavenging activity assay using $p$-cresol as a spectrofluorometric probe

Authors : Burcu BEKDEŞER, Gülüzar ZEYTÜNLÜ, Mustafa ÖZYÜREK, Mustafa Reşat APAK

Pages : 429-438

View : 18 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, a novel spectrofluorometric assay based on $p$-cresol insert ignore into journalissuearticles values(4-methyl phenol); probe is developed for the measurement of HOBr scavenging activity. It is the first study involving the use of a $p$-cresol probe for the determination of the HOBr scavenging activity of biothiols. While the $p$-cresol probe insert ignore into journalissuearticles values($\lambda_{ex}$ = 260 nm, $\lambda_{em}$ = 305 nm); has fluorescence characteristics, its brominated derivatives emerging at the end of the oxidation reaction with HOBr do not show fluorescence. The initial fluorescence intensity of the $p$-cresol probe is decreased in the presence of the brominating agent, HOBr, and this decrease is lower in the presence of HOBr scavenging antioxidants. The scavenging activities of biothiols tested with respect to the developed method decrease in the following order: penicillamine $>$ N-acetyl cysteine $>$ L-glutathione insert ignore into journalissuearticles values(reduced); $>$ cysteamine $>$ homocysteine $>$ glutathione ethyl ester $>$ cysteine $>$ 1,4-dithiothreitol $>$ lipoic acid $>$ methionine. Penicillamine insert ignore into journalissuearticles values(IC$_{50}$ = 10.12 $\mu $M); was the most effective HOBr-scavenger among the tested biothiols. The results obtained with the developed method for biothiols and some pharmaceutical samples were statistically compared insert ignore into journalissuearticles values(using ANOVA); to those found by the reference methods insert ignore into journalissuearticles values(KI/taurine and UPLC);. The advantage of the proposed method over the KI/taurine assay was demonstrated
Keywords : Spectrofluorometric method, hypobromous acid scavenging, p cresol probe, biothiols