Synthesis, spectroscopic characterization, and computational studies of 2-cyano-6-hydroxybenzothiazole: a key synthetic intermediate of firefly luciferin

Authors : Ghasem SHAHMORADI, Saeid AMANI

Pages : 1499-1517

View : 16 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Firefly luciferin is widely applied as a biotechnological tool for visualizing various biological processes in vitro and in vivo. Chemically, 2-cyano-6-hydroxybenzothiazole, as a key synthetic intermediate of firefly luciferin, is obtained from 2-cyano-6-methoxybenzothiazole by changing the methoxy with a hydroxy group. However, this approach is costly and not suited for large-scale synthesis. Here we report cost-effective and efficient syntheses of 2-cyano-6-hydroxybenzothiazole through the catalytic Sandmeyer-type cyanation reaction. Our approach employs diazonium tetrafluoroborate salt of 2-amino-6-hydroxybenzothiazole as a cyanation substrate. The cyanation reaction proceeds efficiently under mild conditions by using Cuinsert ignore into journalissuearticles values(I);/Cuinsert ignore into journalissuearticles values(II);/N,N,N\textquotesingle,N\textquotesingle-tetramethylethylenediamine as a catalyst. In addition, computational studies of the 2-cyano-6-hydroxybenzothiazole structure were performed based on the density functional theory method. The theoretical parameters of the optimized geometry were derived from the B3LYP/6-311$++$Ginsert ignore into journalissuearticles values(d, p); method. Time-dependent density functional theory was applied to assign the electronic absorption bands observed experimentally and the $^{1}$H NMR chemical shifts were computed using the GIAO method. There was a significant relationship between computational studies and experimental results.
Keywords : Firefly luciferin, diazonium tetrafluoroborate, Sandmeyer, 2 cyano 6 hydroxybenzothiazole, computational studies