Preparation of self-propelled Cu-Pt micromotors and their application in miRNA monitoring

Authors : Lütfi ÖKSÜZ, Gözde Yurdabak KARACA, Filiz KURALAY, Emre UYGUN, İsmihan Ümran KOÇ, Ayşegül Uygun ÖKSÜZ

Pages : 1744-1754

View : 22 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Self-propelled catalytic micromotors offer considerable promise in terms of many applications. Catalytic micromotors are strongly influenced by the presence and concentration of specific ions and chemicals in the environment, making them useful as sensors and actuators. In this work, copper insert ignore into journalissuearticles values(Cu);-platinum insert ignore into journalissuearticles values(Pt); micromotors were fabricated by using the magnetron sputtering method for the first time in the literature and their applications based on the detection of miRNA-21 were evaluated. We analyzed the dependence of the mobility of Cu-Pt micromotors using different concentrations of hydrogen peroxide insert ignore into journalissuearticles values(H$_{2}$O$_{2});$. The presence of surfactants in the environment is also important for the movement of the micromotors. Thus, we studied the effect of three different surfactants: anionic as sodium dodecyl sulfate insert ignore into journalissuearticles values(SDS);, cationic as cetyltrimethylammonium bromide insert ignore into journalissuearticles values(CTAB);, and nonionic as Triton X-100. Cu-Pt micromotor motion was observed even at very low concentrations of surfactant insert ignore into journalissuearticles values(0.01%); and hydrogen peroxide insert ignore into journalissuearticles values(0.25%);. miRNAs have been regarded as biomarker candidates in early diagnosis. Our sensing strategy relied on dye-labeled single-stranded DNA immobilization onto Cu-Pt micromotors that recognize the target miRNA-21. The changes in the fluorescence intensity as well as the changes in the speed of micromotors were examined before and after hybridization.
Keywords : Micromotors, self propelled micromotors, magnetron sputtering, miRNA 21