GC-MS analysis of the methanolic extracts of Smilax china and Salix alba and their antioxidant activity

Authors : Abdul QADIR, Mohd AQIL, Athar ALI, Farhan J AHMAD, Sayeed AHMAD, Muhammad ARIF, Nausheen KHAN

Pages : 352-363

Doi:10.3906/kim-1907-5

View : 18 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Smilax china L. familySmilacaceae and Salix alba L. familySalicaceae areplantsthathavebeentraditionally used to treat various ailments in Indian and Chinese medicine. A quantitative estimation of the methanolic extracts of these plants was performed by GC-MS analysis to obtain insight into its phytoconstituents responsible for therapeutic action. Theantioxidantpotentialofthemethanolextractsof Smilax china MESC and Salix alba MESA wereassessed with DPPH by using a UV spectrophotometer at a wavelength of 517 nm. The prevailing compounds found in MESC were lactam sugars including 2,5-dimethyl-2,4-dihydroxy-3 2H -furanon 1.40% , 1,5-anhydro-6-deoxyhexo-2,3-diulose 4.33% , and alpha-methyl-1-sorboside 1.80% ; the two alkaloids found were 1,4-methane-4,4a,5,6,7,8,9,9a-octahydro10,10-dimethyl cyclohepta[d] pyridazine 0.87% and 1,3,7-trimethyl-2,6-dioxopurine 0.54% ; terpenes included deltacadinene 0.39% , terpineol, + -cedrol 22.13% , 3-thujanol 0.77% , and 9,10-dehydro-cycloisolongifolene 0.34% ; fatty acids included cis-vaccenic acid 4.98% and telfairic acid 1.10% ; esters included 1,2,3-propanetriol diacetate 7.56% , 7-hexadecenoic acid, methyl ester 1.77% , eicosanoic acid, and methyl ester 0.95% ; and glycerol included 1,2,3-propanetriol 28.75% . The interesting compounds found in MESA were reducing sugars like D-allose 4.40% and pyrogallol 10.48% , alkaloids like caffeine 63.49% , and esters like methyl octadecanoate 0.53% . Both fractions revealed considerable antioxidant activity. The reported existing phenolic compounds and terpenes are responsible for the antioxidant activity of the plant extracts.
Keywords : Smilax china, Salix alba, total antioxidant potential, DPPH assay, GC MS