Dispersive liquid-liquid microextraction for the isolation and HPLC-DAD determination of three major capsaicinoids in Capsicumannuum L.

Authors : Jude CALEB, Usama ALSHANA, Azmi HANOĞLU, İhsan ÇALIŞ

Pages : 420-429

View : 23 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Dispersive liquid-liquid microextraction insert ignore into journalissuearticles values(DLLME); was combined with high-performance liquid chromatography-diode-array detector insert ignore into journalissuearticles values(HPLC-DAD); for the extraction and quantitation of three major capsaicinoids insert ignore into journalissuearticles values(i.e. capsaicin, dihydrocapsaicin and nordihydrocapsaicin); from pepper insert ignore into journalissuearticles values(Capsicum annuum L.);. Chloroform insert ignore into journalissuearticles values(extraction solvent, 100 μL);, acetonitrile insert ignore into journalissuearticles values(disperser solvent, 1250 μL); and 30 s extraction time were found optimum. The analytes were back-extracted into 300 μL of 50 mM sodium hydroxide/ methanol, 45/55% insert ignore into journalissuearticles values(v/v);, within 15 s before being injected into the instrument. Enrichment factors ranged from 3.3 to 14.7 and limits of detection from 5.0 to 15.0 μg g–1. Coefficients of determination insert ignore into journalissuearticles values(R2); and %RSD were higher than 0.9962 and lower than 7.5%, respectively. The proposed method was efficiently applied for the extraction and quantitation of the three capsaicinoids in six cultivars of Capsicum annuum L. with percentage relative recoveries in the range of 92.0%–108.0%. DLLME was also scaled up for the isolation of the three major capsaicinoids providing purity greater than 98.0% as confirmed by liquid chromatography-mass spectrometry insert ignore into journalissuearticles values(LC-MS); and nuclear magnetic resonance insert ignore into journalissuearticles values(NMR); analysis, which significantly reduced the extraction time and organic solvent consumption.
Keywords : Capsaicin, Capsicum annuum L, dihydrocapsaicin, dispersive liquid liquid microextraction, nordihydrocapsaicin