The application of qNMR for the determination of rosuvastatin in tablet form

Authors : Gokhan DİKMEN, Okan USLU

Pages : 132-142

View : 18 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, quantative nuclear magnetic resonance insert ignore into journalissuearticles values(qNMR); method was used to determine the content of rosuvastatin in tablet. Linearity, range, limit of detection insert ignore into journalissuearticles values(LOD);, limit of quantification insert ignore into journalissuearticles values(LOQ);, accuracy, and precision were determined in validation study of rosuvastatin. Furthermore, validation study of rosuvastatin was performed with high performance liquid chromatography insert ignore into journalissuearticles values(HPLC);. Uncertainties of qNMR and HPLC methods were determined using per EURACHEM/CITAC Guide CG 4 insert ignore into journalissuearticles values(3th edition);, quantifying uncertainty in analytical measurement. qNMR and HPLC methods were linear in the ranges of 0.10 - 5.00 mg/mL and 0.001 - 0.0995 mg/mL, respectively and these lineraties indicate very good linearity performance with regression coefficients insert ignore into journalissuearticles values(R2 value); above > 0.99. Moreover, LOD and LOQ values using qNMR method were observed as 0.25 mg/mL and 0.80 mg/mL, respectively. These values using HPLC method were found as 0.00051 mu g/mL and 0.001695 mu g/mL, respectively. The strengths and weaknesses of qNMR method and HPLC method were determined with spectral emphasis on the role of identical reference standards in qualitive and quantitative analyses. It was found that qNMR method is simple, efficient, reliable, and accurate method. Moreover, qNMR method is an easy, practical, and useful method for the validation and optimization of rosuvastatin in the tablet.
Keywords : qNMR, proton nuclear magnetic resonance, high performance liquid chromatography, rosuvastatin, validation