Flowerlike hybrid horseradish peroxidase nanobiocatalyst for the polymerization of guaiacol

Authors : Ersen GOKTURK

Pages : 1285-1292

View : 20 | Download : 11

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, the catalytic activity and stability of flowerlike hybrid horseradish peroxidase insert ignore into journalissuearticles values(HRP); nanobiocatalyst insert ignore into journalissuearticles values(HRP-Cu2+); obtained from Cu2+ ions and HRP enzyme in the polymerization reaction of guaiacol were analyzed. We demonstrated that HRP-Cu2+ and hydrogen peroxide insert ignore into journalissuearticles values(H2O2); initiator showed significantly increased catalytic activity and stability on the polymerization of guaiacol compared to that of free HRP enzyme. Polyinsert ignore into journalissuearticles values(guaiacol); was observed with quite high yields insert ignore into journalissuearticles values(88%); and molecular weights insert ignore into journalissuearticles values(38,000 g/mol); under p11 7.4 phosphate-buffered saline insert ignore into journalissuearticles values(PBS); conditions at 60 degrees C. with 5 weight% of HRP-Cu2+ loading. HRP-Cu2+ also shows very high thermal stability and works even at 70 degrees C reaction temperature; free HRP enzyme denatures at that temperature. Furthermore, HRP-Cu2+ provided considerable repeated use and showed some degree of catalytic activity, even after the fourth recycle, in the polymerization of guaiacol.
Keywords : Horseradish peroxidase, hybrid nanoflowers, polymerization, guaiacol