Evaluation of a novel nanocrystalline hydroxyapatite powder and a solid hydroxyapatite/Chitosan-Gelatin bioceramic for scaffold preparation used as a bone substitute material

Authors : Sharmin RAHMAN, Kazi Hanium MARİA, Mohammad Saif ISHTİAQUE, Arijun NAHAR, Harinarayan DAS, Sheikh Manjura HOQUE

Pages : 884-900

View : 18 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Artificially fabricated hydroxyapatite insert ignore into journalissuearticles values(HAP); shows excellent biocompatibility with various kinds of cells and tissues which makes it an ideal candidate for a bone substitute material. In this study, hydroxyapatite nanoparticles have been prepared by using the wet chemical precipitation method using calcium nitrate tetra-hydrate [Cainsert ignore into journalissuearticles values(NO3);insert ignore into journalissuearticles values(2);center dot 4Hinsert ignore into journalissuearticles values(2);O] and di-ammonium hydrogen phosphate [insert ignore into journalissuearticles values(NH4);insert ignore into journalissuearticles values(2);HPO4] as precursors. The composite scaffolds have been prepared by a freeze-drying method with hydroxyapatite, chitosan, and gelatin which form a 3D network of interconnected pores. Glutaraldehyde solution has been used in the scaffolds to crosslink the amino groups insert ignore into journalissuearticles values(vertical bar NH2); of gelatin with the aldehyde groups insert ignore into journalissuearticles values( CHO); of chitosan. The X-ray diffraction insert ignore into journalissuearticles values(XRD); performed on different scaffolds indicates that the incorporation of a certain amount of hydroxyapatite has no influence on the chitosan/gelatin network and at the same time, the organic matrix does not affect the crystallinity of hydroxyapatite. Transmission electron microscope insert ignore into journalissuearticles values(TEM); images show the needle-like crystal structure of hydroxyapatite nanoparticle. Scanning Electron Microscope insert ignore into journalissuearticles values(SEM); analysis shows an interconnected porous network in the scaffold where HAP nanoparticles are found to be dispersed in the biopolymer matrix. Fourier transforms infrared spectroscopy insert ignore into journalissuearticles values(FTIR); confirms the presence of hydroxyl group insert ignore into journalissuearticles values(OH-); , phosphate group insert ignore into journalissuearticles values(PO43-);, carbonate group insert ignore into journalissuearticles values(CO32-);, imine group insert ignore into journalissuearticles values(C=N);, etc. TGA reveals the thermal stability of the scaffolds. The cytotoxicity of the scaffolds is examined qualitatively by VERO insert ignore into journalissuearticles values(animal cell); cell and quantitatively by MTT-assay. The MTT-assay suggests keeping the weight percentage of glutaraldehyde solution lower than 0.2%. The result found from this study demonstrated that a proper bone replacing scaffold can be made up by controlling the amount of hydroxyapatite, gelatin, and chitosan which will be biocompatible, biodegradable, and biofriendly for any living organism.
Keywords : Hydroxyapatite, chitosan, gelatin, FTIR, MTT assay