Fluorescent sensing platform for low-cost detection of Cu2+ by coumarin derivative: DFT calculation and practical application in herbal and black tea samples

Authors : Tahir SAVRAN, Abdurrahman KARAGOZ, Sukriye Nihan KARUK ELMAS, Duygu AYDİN, Furkan OZEN, Kenan KORAN, Fatma Nur ARSLAN, Ahmet Orhan GORGULU, Ibrahim YİLMAZ

Pages : 1148-1163

View : 25 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A fluorogenic probe based on a coumarin-derivative for Cu2+ sensing in CH3 CN/H2 O media insert ignore into journalissuearticles values(v/v, 95/5, 5.0 μM); was developed and applied in real samples. 3-insert ignore into journalissuearticles values(4-chlorophenyl);-6,7-dihydroxy-coumarin insert ignore into journalissuearticles values(MCPC); probe was obtained by synthetic methodologies and identified by spectral techniques. The probe MCPC showed remarkable changes with a “turn-off” fluorogenic sensing approach for the monitoring of Cu2+ at 456 nm under an excitation wavelength of 366 nm. The response time of the probe MCPC was founded as only 1 min. The detection limit of the probe MCPC was recorded to be 1.47 nM. The binding constant and possible stoichiometric ratio insert ignore into journalissuearticles values(1:1); values were determined by Benesi-Hildebrand and Job’s plot systems, respectively. The mechanism of the probe MCPC with Cu2+ was further confirmed by ESI-MS and FT-IR analyses, as well as supported by theoretical calculations. Furthermore, the probe MCPC was successfully employed for the practical applications to sense Cu2+ in different herbal and black tea samples. The proposed sensing method was also verified by ICP-OES method.
Keywords : Coumarin, fluorescent sensor, copper, DFT, tea samples