Production and Characterization of Isopropyl Laurate Using Immobilized Lipase

Authors : Gülten ŞEKEROĞLU, Sibel FADILOĞLU and Esra İBANOĞLU

Pages : 241-248

Doi:10.3906/sag-1202-53

View : 16 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Immobilized Candida antarctica lipase insert ignore into journalissuearticles values(Novozym 435); was used for the synthesis of isopropyl laurate from isopropyl alcohol and lauric acid. A molecular sieve was used to shift the reaction towards the synthesis. The maximum enzyme activity was obtained at 60 °C. The amount of product seemed to be linearly proportional to the enzyme concentration up to 50 mg. A linear increase in the amount of isopropyl laurate was observed throughout the 50 min reaction. Product formation followed a linear increase up to a lauric acid concentration of 100 mg. Then product formation slowed down. The hydrophile-lipophile balance insert ignore into journalissuearticles values(HLB); value of isopropyl laurate was calculated as 3.5 and its critical micelle concentration insert ignore into journalissuearticles values(CMC); value was found to be 0.684 mM and 0.687 mM from the conductivity and surface tension curve, respectively.
Keywords : Candida antarctica lipase, Enzymatic esterification, Isopropyl laurate, Surfactant