Use of response surface methodology for the bioaccumulation of Violet 90 metal-complex dye by Candida tropicalis

Authors : MÜJGAN OKUR, NURDAN SARAÇOĞLU, ZÜMRİYE AKSU

Pages : 217-230

Doi:10.3906/muh-1409-2

View : 17 | Download : 15

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, the combined effects of initial dye insert ignore into journalissuearticles values(40-700 mg/L); and initial reducing sugar concentration insert ignore into journalissuearticles values(1-15 g/L); on the bioaccumulation of Violet 90 metal-complex dye by growing cells of Candida tropicalis yeast were investigated in growth media containing sugar beet molasses as a carbon and energy source. The bioaccumulation experiments were performed at pH 3 at 30 $^{\circ}$C and at 140 rpm agitation rate in a batch system. The highest uptake was obtained as 61.46% at 14.9 g/L reducing sugar concentration and at 38 mg/L dye concentration while the maximum uptake capacity was achieved as 56.28 mg/g at 3.2 g/L reducing sugar concentration and at 711.1 mg/L dye concentration. Higher uptakes were obtained at lower dye concentrations; higher uptake capacities were observed at higher initial dye concentrations. The combined effects of the initial dye insert ignore into journalissuearticles values(100-500 mg/L); and initial reducing sugar concentration insert ignore into journalissuearticles values(5-15 g/L); on dye uptake capacity and growth rate of Candida tropicalis yeast were also investigated by response surface methodology insert ignore into journalissuearticles values(RSM);. Optimum design variables from RSM were calculated by numerical optimization with the Design Expert program. The optimum values of the variables to maximize uptake capacity were estimated as 5.1 g/L reducing sugar concentration and 499 mg/L dye concentration. The maximum uptake capacity was achieved as 41.3 mg/g at these optimized conditions.
Keywords : Bioaccumulation, metal complex dye, yeast, sugar beet molasses, Candida tropicalis