The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii

Authors : Kader YILDIZ, Oğuz KUL, Sami GÖKPINAR, Hasan Tarık ATMACA, Yılmaz Emre GENÇAY, Aycan Nuriye GAZYAĞCI, Cahit BABÜR, İsmayil Safa GÜRCAN

Pages : 169-175

Doi:10.3906/sag-1301-9

View : 23 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Skeletal muscles insert ignore into journalissuearticles values(tongue, masseter, leg, intercostal, and diaphragmatic muscles); and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray insert ignore into journalissuearticles values(TMA); technique with immunoperoxidase staining were used. Seropositivity was detected in 88% insert ignore into journalissuearticles values(88/100); of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 insert ignore into journalissuearticles values(52.3%, 46/88); and 15 insert ignore into journalissuearticles values(17%, 15/88); of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25–58 × 25–62 insert ignore into journalissuearticles values(mean 34 × 36); µm. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 insert ignore into journalissuearticles values(P < 0.01); and at 1/64 and 1/128 insert ignore into journalissuearticles values(P < 0.001); serum dilutions.
Keywords : Key words Toxoplasma gondii, tissue cyst, percoll gradient centrifugation, tissue microarray, serology, sheep, Turkey