Evaluating the effects of chitosan, plant oils, and different diets on rumen metabolism and protozoan population in sheep

Authors : Monika WENCELOVA, Zora VÁRADYOVA, Katarína MIHALIKOVA

Pages : 26-33

Doi:10.3906/vet-1307-19

View : 19 | Download : 11

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Chitosan insert ignore into journalissuearticles values(CH); has been shown to be a promising natural antimicrobial agent. This study examined the effects on rumen metabolism when providing diets containing high forage insert ignore into journalissuearticles values(HFD);, low forage insert ignore into journalissuearticles values(LFD);, and maize silage insert ignore into journalissuearticles values(MSD); to a 24-h batch culture in which the diets were not supplemented insert ignore into journalissuearticles values(control); or were supplemented with CH insert ignore into journalissuearticles values(100 mg/L);, sunflower oil insert ignore into journalissuearticles values(SF, 35.0 g/kg dry matter);, rapeseed oil insert ignore into journalissuearticles values(RP, 35.0 g/kg dry matter);, or SF and RF with chitosan combinations insert ignore into journalissuearticles values(SFCH and RPCH);. Dry matter digestibility in the batch culture was influenced by CH and was decreased in HFD compared with the control insert ignore into journalissuearticles values(P < 0.01);. Decreasing total number of protozoa and the number of Entodinium spp. was evident, especially in LFD and MSD, with all additives. RPCH had a predominantly adverse effect on Dasytricha ruminantium in MSD. Interactions of the diets × additives in the majority of rumen ciliate populations were detected. The concentrations of trans-vaccenic acid and conjugated linoleic acid insert ignore into journalissuearticles values(c9, t11-CLA); were unchanged by chitosan. However, the combination of SF and RF with chitosan insert ignore into journalissuearticles values(SFCH and RPCH); positively affected fatty acid profiles, which might be related to differences in diets, SF or RP composition, and different microbial activity in the batch cultures.
Keywords : sunflower oil, rapeseed oil, rumen ciliate protozoa, fatty acids, Chitosan