Comparison of the ovine prion protein genotype profiles of breeds Tigaie with Black Head and Merino

Authors : Stelian BARAITAREANU, Maria Rodica OTELEA, Mihaela ZAULET, Kurt SCHNEIDER, Mihai TURCITU, Leonardo LEONARDI, Doina DANES

Pages : 625-630

Doi:10.3906/vet-1302-50

View : 23 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A comparison between a rare old breed and a common breed of ovine prion protein insert ignore into journalissuearticles values(PrP); genotype profiles was performed in 3 Romanian sheep flocks. The total number of animals used in the research was 698 sheep, of which 53 were Merino breed insert ignore into journalissuearticles values(MB); rams, 12 were Tigaie with Black Head breed insert ignore into journalissuearticles values(TBHB); rams, 340 were MB ewes, and 293 were TBHB ewes. The PrP genotype was investigated by identification of codons 136, 154, and 171 and by gene sequencing between codons 101 and 227. All sheep groups contained the ARR, AHQ, ARH, ARQ, and VRQ haplotypes, with frequencies ranging from 1.37% insert ignore into journalissuearticles values(ARH in TBHB ewes); to 51.71% insert ignore into journalissuearticles values(ARQ in TBHB ewes);. TBHB had a low prevalence of AHQ insert ignore into journalissuearticles values(2.05% in ewes, 4.17% in rams);, ARH insert ignore into journalissuearticles values(1.37% in ewes, 4.17% in rams);, and VRQ insert ignore into journalissuearticles values(1.88% in ewes, 8.33% in rams);. The highest haplotype in TBHB was ARQ insert ignore into journalissuearticles values(51.71% in ewes, 37.50% in rams);. ARQ was the most frequent haplotype in the MB insert ignore into journalissuearticles values(49.41% in ewes, 42.45% in rams);. MB had a low prevalence of AHQ insert ignore into journalissuearticles values(3.24% in ewes, 6.60% in rams);, ARH insert ignore into journalissuearticles values(1.47% in ewes, 5.66% in rams);, and VRQ insert ignore into journalissuearticles values(3.53% in ewes, 9.43% in rams);. The results indicated that 66.66% of TBHB rams and 68.26% of TBHB ewes have scrapie-resistant genotypes, despite the absence of a genetic selection program for scrapie. The proportion of scrapie resistant alleles was higher in the TBHB rams than in the MB rams.
Keywords : Key words TSEs, PrP genotyping, scrapie