Polymorphism of 5’ regulatory region of caprine FSHR gene and its association with litter size in Jining Grey goat

Authors : Xiaohong GUO, Yang LI, Mingxing CHU, Chunjing FENG, Ran DI, Qiuyue LIU, Tao FENG, Guiling CAO, Dongwei HUANG, Li FANG, Qianqian TANG, Yuehui MA, Kui LI

Pages : 497-503

Doi:10.3906/tar-1209-52

View : 21 | Download : 11

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Single nucleotide polymorphisms of the 5` regulatory region of the FSHR gene were detected in 3 goat breeds insert ignore into journalissuearticles values(Jining Grey, Boer, and Inner Mongolia Cashmere); by the polymerase chain reaction-single-strand conformation polymorphism method, and its effects on litter size in Jining Grey goats were also evaluated. Concerning primer P1, 2 genotypes insert ignore into journalissuearticles values(AA and BB); were detected in Boer goats, while only the AA genotype was identified in Jining Grey and Inner Mongolia Cashmere goats. Sequencing revealed a T->A transversion at the 26th position and an A->C transversion at the 61st position of the amplified region in genotype BB in comparison to genotype AA. Regarding primer P2, 3 genotypes insert ignore into journalissuearticles values(CC, CD, and DD); were identified in the 3 goat breeds mentioned above. Sequencing revealed a T->A transversion at the 70th position and a G->C transversion at the 130th position of the amplified region in genotype DD compared with CC. Does with genotype CC had 0.46 insert ignore into journalissuearticles values(P < 0.05); or 1.03 insert ignore into journalissuearticles values(P < 0.05); kids more than those with genotype CD or DD, respectively, while does with CD had 0.57 insert ignore into journalissuearticles values(P < 0.05); kids more than those with DD in Jining Grey goats. These results preliminarily showed that allele C of the FSHR gene was a potential marker for improving litter size in goats.
Keywords : Key words Goat, litter size, FSHR gene, polymerase chain reaction single strand conformation polymorphism