Cytotoxicity of nickel ions for human osteoblasts in the context of orthodontic treatment in humans and animals

Authors : Marcin MIKULEWICZ, Katarzyna CHOJNACKA, Iwona KOCHANOWSKA, Agnieszka DZIEWICKA, Maciej JANECZEK, Jerzy CIEPLIK, Aleksander CHRÓSZCZ

Pages : 164-169

Doi:10.3906/vet-1112-34

View : 17 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In the present work, the biocompatibility of orthodontic devices was tested by the propagation of human osteoblasts. A different methodological approach was applied. Since the materials used in human and veterinary orthodontics insert ignore into journalissuearticles values(not in implantology); had been tested, osteoblasts were not implanted on the surface of the biomaterial, but have been cultured in the medium containing the products of corrosion. In the present work, osteoblasts were propagated in the medium spiked with Niinsert ignore into journalissuearticles values(II); salt versus the control. Osteoblast cells insert ignore into journalissuearticles values(live, dead, and total); were counted under light microscope. The statistical significance of the differences between the experimental and control groups were evaluated insert ignore into journalissuearticles values(P < 0.05);. Based on the number of live, dead, and total cells, the viability and proliferation were determined. A slight cytotoxic effect of Niinsert ignore into journalissuearticles values(II); ions at the studied concentration was confirmed. The mean viability was 7% lower in the experimental versus the control group insert ignore into journalissuearticles values(the difference was statistically significant);, while the change of proliferation was not significant.
Keywords : Key words Nickel II, cytotoxicity, osteoblasts, orthodontic appliance