The effects of oviductal cell co-culture and different gas mixtures on the development of bovine embryos in vitro

Authors : Özen Banu ÖZDAŞ, Ümüt CİRİT, Alper BARAN, Güven KAŞIKÇI

Pages : 362-366

View : 16 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The objective of the current study was to investigate the effects of the addition of oviduct co-culture to synthetic oviduct fluid insert ignore into journalissuearticles values(SOF); and Ménézo B2 media used in an in vitro study of cows, and the effects of different gas atmospheres insert ignore into journalissuearticles values(5% CO2 or 5% CO2, 5% O2, and 90% N2); on in vitro cultures. Oocytes obtained through aspiration from the ovaries of slaughtered Holstein cows were washed and cultured within a TCM-199 maturation medium at 38.8 °C for 23 h. Then the matured oocytes and thawed semen, prepared in accordance with the swim-up method, were incubated for fertilization within in vitro fertilization insert ignore into journalissuearticles values(IVF);-Tyrode`s albumin lactate pyruvate insert ignore into journalissuearticles values(TALP); medium with an atmosphere including the combination of 5% CO2, 5% O2, and 90% N2 gas, at 38.8 °C for 18-24 h. Fertilized oocytes were distributed into 3 main groups: Group I: B2 insert ignore into journalissuearticles values(5% CO2);, Group II: B2 medium insert ignore into journalissuearticles values(5% CO2, 5% O2, and 90% N2);, and Group III: SOF insert ignore into journalissuearticles values(5% CO2, 5% O2, and 90% N2);, and each group was divided into 2 subgroups, with and without oviduct co-culture cells, and were cultured for 9 days. The percentage of embryos that reached the blastocyst stage was 34.0%, 20.0%, and 32.3% in the co-culture group and 26.7% and 23.3% in the without co-culture group, respectively. Group III showed the highest development of expanded blastocyst stage insert ignore into journalissuearticles values(P < 0.01);. In conclusion, the addition of co-culture to B2 insert ignore into journalissuearticles values(5% CO2 atmosphere); and SOF insert ignore into journalissuearticles values(5% CO2, 5% O2, and 90% N2 atmosphere); media increased the rate of transferable embryos.
Keywords : Key words Cattle, embryo, oviduct co culture, in vitro, gas mixture