Protective effect of N-acetyl-L-cysteine against acrylamide-induced oxidative stress in rats

Authors : Ebru Işık ALTURFAN, Ayfer BECEREN, Ahmet Özer ŞEHİRLİ, Zeynep Emel DEMİRALP, Göksel ŞENER, Gülden Zehra OMURTAG

Pages : 438-445

Doi:10.3906/sag-1207-71

View : 17 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Acrylamide insert ignore into journalissuearticles values(AA);, used in many fields, from industrial manufacturing to laboratory work, is also formed during the heating process through the interactions of amino acids. Therefore, AA poses a significant risk for both human and animal health. This study aimed to elucidate whether N-acetyl-L-cysteine insert ignore into journalissuearticles values(NAC); treatment could modulate AA-induced oxidative changes in the brain, lung, liver, kidney, and testes tissues of the rat. Rats were divided into 4 groups, as the control insert ignore into journalissuearticles values(C);, NAC [150 mg/kg intraperitoneally insert ignore into journalissuearticles values(i.p.);], AA insert ignore into journalissuearticles values(40 mg/kg i.p.);, and NAC + AA groups. After 10 days, the rats were decapitated and the tissues were excised. Malondialdehyde insert ignore into journalissuearticles values(MDA); and glutathione insert ignore into journalissuearticles values(GSH); levels, and myeloperoxidase activity insert ignore into journalissuearticles values(MPO); were determined in the tissues, while oxidant-induced tissue fibrosis was determined using the collagen contents. Serum enzyme activities, cytokine levels, and leukocyte apoptosis were assayed in the plasma. In the AA group, GSH levels decreased significantly, while the MDA levels, MPO activity, and collagen content increased in the tissues suggesting oxidative organ damage. In the NAC + AA group, oxidant responses reversed significantly. Serum enzyme activities, cytokine levels, and leukocyte apoptosis, which increased following AA administration, decreased with NAC treatment. Therefore, supplementing with NAC can be useful when there is a risk of AA toxicity, as NAC inhibits neutrophil infiltration, balances the oxidant-antioxidant status, and regulates the generation of inflammatory mediators to protect tissues.
Keywords : Key words Acrylamide, N acetyl L cysteine, oxidative changes, collagen, myeloperoxidase, leukocyte apoptosis