The detection of classical enterotoxins of Staphylococcus aureus in raw cow milk using the ELISA method

Authors : Ebrahim RAHIMI, Hassan MOMMTAZ, Amir SHAKERIAN, Hamid Reza KAVYANI

Pages : 319-322

View : 16 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Milk and dairy products are frequently contaminated with exterotoxigenic Staphylococcus aureus, which is often involved in Staphylococcal food poisoning. The aim of the present study is to investigate the presence of classical enterotoxins of S. aureus in raw milk that was produced in Isfahan, Iran. For this purpose, 72 milk samples were collected from the bulk milk tanks of 12 milking farms during 3 different seasons insert ignore into journalissuearticles values(winter, spring, and summer); and tested for the presence of staphylococcal enterotoxins insert ignore into journalissuearticles values(SEs); using the enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA); method. Out of 72 samples studied, 15 samples insert ignore into journalissuearticles values(20.8%); were positive for at least 1 SE. Of these, 12 insert ignore into journalissuearticles values(16.7%); were positive for SEA, 9 insert ignore into journalissuearticles values(12.5%); for SED, and 6 insert ignore into journalissuearticles values(8.3%); for SEC. None of the samples was positive for SEB or SEE. Statistical evaluation showed that there were not any significant differences insert ignore into journalissuearticles values(P > 0.05); between the presence of SEs in the milk samples tested in winter, spring, and summer. Further studies should be carried out to investigate the presence of these toxins in different foods and their roles in food poisoning.
Keywords : Key words Enterotoxins, milk, Staphylococcus aureus