Infectious pancreatic necrosis in a recirculating rainbow trout (Oncorhynchus mykiss) culture system

Authors : Nasrollah AHMADI, Mostafa AKHLAGHI, Ahmad ORYAN, Arsalan HOSSEINI

Pages : 303-309

View : 19 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :During October 2009, a suspected outbreak of infectious pancreatic necrosis, with typical signs and high mortality, was observed in cultured rainbow trout insert ignore into journalissuearticles values(Oncorhynchus mykiss); fry insert ignore into journalissuearticles values(mean weight of 9 g); in a recirculating system in Fars Province, Iran. Tissue samples, including pyloric caeca as well as pancreatic tissues, liver, kidney, and spleen, were aseptically collected from 20 affected moribund fish for histopathological and molecular assays. Histopathologically, the exocrine pancreatic acinar cells exhibited the most notable pathology, including focal to extensive degeneration and coagulative necrosis with mononuclear inflammatory infiltrates. To confirm the diagnosis of clinical infectious pancreatic necrosis insert ignore into journalissuearticles values(IPN);, a reverse transcription-polymerase chain reaction insert ignore into journalissuearticles values(RT-PCR); and sequence analysis were used. A 224-bp fragment of the IPN virus NS/VP3 insert ignore into journalissuearticles values(segment A); gene was amplified using cDNA prepared from infected pooled tissues of pancreas, liver, kidney, and spleen. The nucleotide sequence was most closely identified with the Sp serotype.
Keywords : Key words Infectious pancreatic necrosis, rainbow trout, recirculating system, histopathology, RT PCR