Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Authors : YongJun LI, Hong AO, Guijin SUN, Li ZHANG

Pages : 15-19

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase insert ignore into journalissuearticles values(PAD);, bovine insulin-like growth factor binding protein-3 insert ignore into journalissuearticles values(IGFBP3);, bovine NADH dehydrogenase insert ignore into journalissuearticles values(ubiquinone); 1 alpha subcomplex 4 insert ignore into journalissuearticles values(NDUFA4);, and dog cyclin B3 insert ignore into journalissuearticles values(CCNB3); genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.
Keywords : Key words Single preimplantation embryo mRNA differential display, goat, embryo, gene