Association of polymorphisms in the intron 1 of duck prolactin with egg performance

Authors : HuiFang LI, WenQi ZHU, KuanWei CHEN, TangJie ZHANG, WeiTao SONG

Pages : 193-197

View : 20 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Gaoyou duck insert ignore into journalissuearticles values(Chinese indigenous breed); is famous for double-yolked egg. In the present study, SNP of prolactin insert ignore into journalissuearticles values(PRL); gene intron 1 of Gaoyou duck was detected by PCR-RFLP and DNA sequencing. The results indicated that there were 2 Dra I recognition sites in intron 1 of the PRL gene, but only 1 was of polymorphism. A T/C mutation at the position of the 1326 bp of the PRL gene was found by DNA sequencing, and resulted in 3 genotypes AA, AB, and BB. The frequencies of genotype BB and allele B were the highest. The association analysis between the polymorphism PRL gene intron 1 and egg performance was carried out. The least square analysis showed that the BB ducks had significant egg weight at the age of 30 weeks than AB ducks insert ignore into journalissuearticles values(P < 0.01);, the double-yolk percentage of the AB ducks was much higher than that of BB ducks insert ignore into journalissuearticles values(P < 0.05); in double-yolk ducks, but there was not significant difference in egg number, longest clutch days, and the body weight at first egg among the three genotypes.
Keywords : PRL gene, PCR RFLP, Gaoyou duck, double yolked egg